A combination of simvastatin and methylprednisolone improves the quality of vitrified-warmed ovarian tissue after auto-transplantation

辛伐他汀和甲基泼尼松龙联合使用可改善自体移植后玻璃化加热卵巢组织的质量

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作者:Jaewang Lee, Eun Jung Kim, Hyun Sun Kong, Hye Won Youm, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim

Methods

Whole ovaries were removed from the mice and vitrified by two-step vitrification procedures. The vitrified ovaries were warmed 1 week later and auto-transplanted under the bilateral kidney capsules. The ovaries and blood samples were collected 2, 7 and 21 days (D) after transplantation for histological analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay, immunohistochemistry for CD31 and serum anti-Mullerian hormone (AMH) level estimation. Embryonic development was evaluated after IVF of oocytes obtained from the transplanted ovary. Main

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