Laboratory and Semi-Field Cage Demography Studies of Diachasmimorpha longicaudata Mass-Reared on Two Ceratitis capitata Strains

对以两种地中海实蝇品系为食的长尾拟步甲进行大规模饲养的实验室和半野外笼养种群统计学研究

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Abstract

Ceratitis capitata (Wiedemann) or medfly is a polyphagous pest of fruit crops worldwide. The Asian-native larval parasitoid Diachasmimorpha longicaudata (Ashmead) is mass-reared at the San Juan Biofactory and is currently released for medfly control in Argentina. Information on parasitoid survival, reproduction, and population growth parameters is critical for optimizing the mass-rearing process and successfully achieving large-scale release. This study provides a first-time insight into the demography of two population lines of D. longicaudata: one mass-reared on medfly larvae of the Vienna-8 temperature-sensitive lethal genetic sexing strain and the other on larvae of the wild biparental medfly strain. The aim was to compare both parasitoid populations to improve mass-rearing quality and to assess performance on medfly in a semi-arid environment, typical of Argentina's central-western fruit-growing region. Tests were performed under laboratory and non-controlled environmental conditions in semi-field cages during three seasons. Dl((Cc-bip)) females exhibited higher reproductive potential than did Dl((Cc-tsl)) females under lab conditions. However, both Dl((Cc-bip)) and Dl((Cc-tsl)) were found to be similar high-quality females with high population growth rates in warm-temperate seasons, i.e., late spring and summer. Dl((Cc-bip)) females were only able to sustain low reproductive rates in early autumn, a colder season. These results are useful for improving the parasitoid mass production at the San Juan Biofactory and redesigning parasitoid release schedules in Argentina's irrigated, semi-arid, fruit-growing regions.

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