Abstract
This study aimed to investigate white matter microstructural changes in patients with cluster headaches using peak width of skeletonized mean diffusivity (PSMD) derived from diffusion tensor imaging (DTI). A total of 40 participants were recruited, comprising 20 patients with newly diagnosed cluster headaches and 20 healthy controls. DTI was conducted using a 3 Tesla MRI scanner. PSMD was quantified through a standardized multi-step process involving preprocessing, skeletonization, application of a custom mask, and histogram analysis performed with the FSL program. PSMD values were compared between the cluster headache group and the healthy controls, and correlation analyses were performed to examine association between PSMD and clinical factors. Patients with cluster headaches demonstrated significantly higher PSMD values than healthy controls (0.273 × 10(⁻3) mm²/s vs. 0.227 × 10(⁻3) mm²/s, p = 0.009). PSMD showed a positive correlation with age in the cluster headache group (r = 0.575, p = 0.008). However, there was no significant difference in PSMD between the hemisphere corresponding to the fisymptomatic side of the cluster headache and the contralateral hemisphere (0.270 × 10(⁻3) mm²/s vs. 0.272 × 10(⁻3) mm²/s, p = 0.909). This study demonstrates that patients with cluster headaches experience compromised white matter microstructural integrity, reflected by elevated PSMD values compared to healthy controls. The observed age-related increase in PSMD across both groups supports the role of aging in white matter microstructure. The lack of hemispheric differences in PSMD suggests that white matter alterations associated with cluster headaches are not confined to the symptomatic side, indicating a diffuse pattern of white matter involvement.