Green fluorescent protein as a reporter to monitor gene expression and food colonization by Aspergillus flavus

绿色荧光蛋白作为报告基因,用于监测黄曲霉的基因表达和食物定殖情况。

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Abstract

Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants.

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