Abstract
Toxicity tests that last the all life duration of the organisms are not common, instead, long-term tests usually include one reproductive cycle. In the present study we optimized and propose a lifespan (all life) term test using Enchytraeus crypticus (Oligochaeta). The effect of copper oxide nanoparticles (CuO-NPs) was assessed in this lifespan test and compared to copper salt (CuCl(2)), using the same effect concentrations on reproduction (EC(50)). Monitored endpoints included survival and reproduction over-time (202 days). Results from survival showed that CuO-NPs caused shorter life of the adults compared to CuCl(2) (control LT(50): 218 days > CuCl(2) LT(50): 175 days > CuO-NPs LT(50): 145 days). The effect was even more amplified in terms of reproduction (control ET(50): 158 days > CuCl(2) ET(50): 138 days > CuO-NPs ET(50): 92 days). Results suggest that CuO-NPs may cause a higher Cu effect via a trojan horse mechanism. The use of lifespan tests brings a novel concept in soil ecotoxicity, the longevity. This is a particularly important aspect when the subject is nanomaterials toxicity, where longer term exposure time is expected to reveal unpredicted effects via the current short/long-term tests. The present study confirms this higher effect for CuO-NPs.