Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food

厄瓜多尔制成品食品中转基因成分检测与定量的三种 DNA 提取方法比较

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作者:Ricardo Pacheco Coello, Jorge Pestana Justo, Andrés Factos Mendoza, Efrén Santos Ordoñez

Results

Twenty-six processed food samples were positive for the P35S alone and eight samples for the Tnos and P35S. Absolute qPCR results indicated that eleven samples were positive for GTS 40-3-2 specific event and two for MON810 specific event. A total of nine samples for events GTS 40-3-2 and MON810 exceeded the umbral allowed of transgenic content in the whole food product with the specific events. Different food products may require different DNA extraction protocols for GMO detection through PCR. Among the three methods tested, the DNeasy mericon food kit DNA extraction method obtained higher proportion of amplified endogenous genes through PCR. Finally, event-specific GMOs were detected in food products in Ecuador.

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