Abstract
Elastic fibers form vessel walls, and elastic fiber calcification causes serious vascular diseases. Elastin is a well-known elastic fiber component; however, the insoluble nature of elastic fibers renders elastic fiber component analysis difficult. A previous study investigated F-box and WD-40 domain-containing protein 2 (FBXW2) in the cambium layer of bovine periosteum and hypothesized that fiber structures of FBXW2 are coated with osteocalcin during explant culture. Here, FBXW2 was expressed around some endothelial cells but not in all microvessels of the bovine periosteum. The author hypothesized that FBXW2 is expressed only in blood vessels with elastic fibers. Immunostaining and Elastica van Gieson staining indicated that FBXW2 was expressed in the same regions as elastic fibers and elastin in the cambium layer of the periosteum. Alpha-smooth muscle actin (αSMA) was expressed in microvessels and periosteum-derived cells. Immunostaining and observation of microvessels with serial sections revealed that osteocalcin was not expressed around blood vessels at 6 and 7 weeks. However, blood vessels and periosteum connoted elastic fibers, FBXW2, and αSMA. These findings are expected to clarify the processes involved in the calcification of elastic fibers in blood vessels.