Abstract
AIM: Composite resins and bonding agents are indispensable in orthodontic practice, necessitating a thorough understanding of their cytotoxic effects, particularly when expired. This study aimed to evaluate the influence of expired composite and bonding materials on human gingival fibroblasts (hGFs). MATERIALS AND METHODS: Both expired and nonexpired composite resins and bonding agents were tested on hGFs using direct exposure methods. Viability assays, morphological evaluations, and wound healing assays were conducted at 24 and 72 hours post exposure. RESULTS: Exposure to both expired and nonexpired materials led to significant reductions in hGF viability and alterations in morphology. Wound healing assays demonstrated impaired migratory abilities of hGFs following exposure to these materials. CONCLUSION: The study highlights the importance of prudent material selection, handling, and monitoring in orthodontic practice to mitigate adverse effects on gingival tissues. Proper management of expired materials is crucial for cost-effectiveness, waste reduction, and patient safety. Further research into the long-term impacts of expired materials on gingival and periodontal health is imperative to ensure clinical treatment safety and efficacy. This investigation provides valuable insights into the biocompatibility of orthodontic bonding materials and emphasizes the necessity for continued vigilance in their usage to uphold patient welfare and treatment quality.