Abstract
Sulfadiazine (SD) residues pose threats to ecosystems and public health, necessitating precise detection and efficient degradation. This study synthesized a cobalt-doped metal-organic framework (Co/ZIF-8) nanocatalyst with a structure mimicking human carbonic anhydrase II (hCAII) through the synergistic reaction of Zn(2+) and Co(2+), exhibiting a Michaelis constant of 0.11 mM. This nanozyme enables both highly sensitive colorimetric detection of SD (linear range: 6.3-950.0 μM, detection limit: 3.2 μM) and activation of H(2)O(2) to generate ·OH for SD degradation, achieving complete degradation of 10 mg/L SD within 35 min at pH 5.0. The nanozyme exhibits excellent cyclic stability, demonstrating reliable SD recovery in real-world samples such as milk and lake water. By integrating SD detection with remediation technology, it provides a practical platform for addressing SD contamination in environmental and food contexts.