Abstract
Loss of T-cell tolerance to multiple islet antigens is a key feature of autoimmune type 1 diabetes. In this study, we investigated the requirement for programmed death 1 (PD-1) expression by CD4(+) T cells in the maintenance of self-tolerance via bystander suppression of autoreactive CD8(+) T cells using nonobese diabetic mice. We used CRISPR/Cas9 to selectively knockout PD-1 in islet antigen-specific BDC2.5 CD4(+) T cells and observed the impact on bystander tolerance of 8.3 CD8(+) T cells, specific for a different islet antigen. Loss of PD-1 promoted the proliferation, Th1-like effector-memory phenotype, islet infiltration and expression of cytotoxic markers by BDC2.5 cells. PD-1-deficient BDC2.5 cells were impaired in their regulation of 8.3 cells, which proliferated more, developed an effector-memory phenotype and increased expression of effector molecules. While antigen-presenting cell maturation and migration into the pancreatic lymph node were not impacted by loss of PD-1 expression from BDC2.5 cells, migration of BDC2.5 cells out of the lymph node was required for enhanced activation of the CD8(+) T cells. Together, these events led to accelerated diabetes progression, suggesting that PD-1 expression by CD4(+) T cells promotes a tolerogenic microenvironment and restraining autoreactive CD8(+) T cells.