Abstract
BACKGROUND: Intracerebral hemorrhage (ICH) is an extremely common acute vascular disease in the elderly population with a high potential for disability and death. OBJECTIVE: To analyze TYROBP related to blood-brain barrier (BBB) function in ICH through online databases, and to explore their mechanisms of action. MATERIALS AND METHODS: The GSE24265 dataset was chosen for GEO2R analysis, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses. It was discovered that TYROBP was related to the "maintenance of permeability of BBB". Subsequently, ICH rat models were constructed, and TYROBP expression in ICH was verified. Lentivirus vectors with abnormally expressed TYROBP were constructed and intervened in ICH rats. The cognitive and learning abilities of rats were tested by the Morris water maze test, and the pathological damage, inflammatory reactions, and oxidative stress responses of brain tissue were detected. Finally, the BBB and microglial pyroptosis in rats were evaluated. RESULTS: Analysis of the GSE24265 dataset showed upregulation of TYROBP, which was also elevated in ICH rats. After enhancing TYROBP expression, the learning and cognitive abilities of ICH rats were further deteriorated, the pathological damage of brain tissues was aggravated, and the BBB leakage and microglial pyroptosis were intensified; silencing TYROBP expression led to completely reversed pathological processes. CONCLUSIONS: TYROBP promotes the pathological progression of ICH by increasing the BBB permeability and facilitating microglial pyroptosis.