Lysozyme orientation and conformation on MoS(2) surface: Insights from molecular simulations

溶菌酶在MoS(2)表面的取向和构象:来自分子模拟的启示

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Abstract

Two-dimensional molybdenum disulfide (MoS(2)) has attracted intense interest owing to its unique properties and promising biosensor applications. To develop effective biocompatible platforms, it is crucial to understand the interactions between MoS(2) and biological molecules such as proteins, but little knowledge exists on the orientation and conformation of proteins on the MoS(2) surface at the molecular level. In this work, the lysozyme adsorption on the MoS(2) surface was studied by molecular dynamics simulations, wherein six different orientations were selected based on the different faces of lysozyme. Simulation results showed that lysozyme tends to adsorb on the MoS(2) surface in an "end-on" orientation, indicating that orientations within this range are favorable for stable adsorption. The end-on orientation could be further categorized into "bottom end-on" and "top end-on" orientations. The driving forces responsible for the adsorption were dominated by van der Waals interactions and supplemented by electrostatic interactions. Further, the conformations of the lysozyme adsorbed on the MoS(2) surface were basically preserved. This simulation study promotes the fundamental understanding of interactions between MoS(2) and proteins and can guide the development of future biomedical applications of MoS(2).

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