Abstract
Brassinosteroids (BRs) are plant steroid hormones involved in plant growth and environmental adaptation. It is well known that oxidation/hydroxylation steps in the BR biosynthetic pathway are catalyzed by cytochrome P450 enzymes. It has been proposed that brassinolide is biosynthesized from campesterol via campestanol (CN) in the original BR biosynthetic pathway. However, a recent enzymatic analysis of cytochrome P450 enzymes and re-evaluation of the endogenous amount of BRs in BR-deficient mutants included an investigation of the novel BR biosynthetic pathway (CN-independent pathway) not via CN. This review highlights comprehensive recent advances in the biochemical research of BR biosynthetic enzymes and the CN-independent pathway. This review also focuses the biosynthesis inhibitors and the antagonists/agonists that are utilized not only as plant growth regulators but also as tools for the chemical and biological investigation of the physiological functions of BRs.