Comparative genomic analysis of Chinese Erwinia amylovora strains reveals genetic variations, plasmid diversity, and potential common ancestor

对中国梨火疫病菌菌株的比较基因组分析揭示了遗传变异、质粒多样性和潜在的共同祖先

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Abstract

The bacterial pathogen Erwinia amylovora is the causative agent of fire blight, a devastating disease affecting rosaceous plants globally. Pathogenicity tests in immature pears demonstrated E. amylovora discovered in China exhibit different virulences. Through full-genome sequencing and genetic variations assay, Each strain exhibited a chromosome size of approximately 3.8 Mb, with distinct distribution patterns in plasmids. The genomes of all sequenced strains were compared to the model strain CFBP1430. Single-nucleotide polymorphisms (SNPs) and short deletions, insertions, and other polymorphisms (DIPs) were identified and shared SNPs and DIPs among strains suggest genomic homogeneity, with a higher level of specificity in DIPs. Phylogenetic analysis inferred that the primary pathway of fire blight spread to China followed a trajectory from the Middle East to Central Asia, ultimately reaching Xinjiang.

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