Conclusion
The study is novel in being a POCT approach for selective determination of H2S molecular form in plasma after simple optimized sample treatment. The study confirms that MI is associated with H2S elevated levels up to 10 hours from emergence of symptoms.
Methods
Dansyl azide was synthesized to react with hydrogen sulfide in biological matrix to produce the fluorescent dansyl amide. Fluorescence was measured at λex 340 nm and λem 517 nm in the new in-house POCT portable spectrofluorometer. The method was validated according to ICH guidelines. Several blood sample treatments and reaction protocols were compared to achieve maximum fluorescence yield.
Results
The H2S Analyser was verified in comparison to a benchtop spectrofluorometer where linearity was confirmed in the range of 3-300 μM, LOD being 1 μM, at λex 340 nm and λem 517 nm. Sample treatment involving blood centrifugation followed by addition of reagent on plasma produced maximum fluorescence yield. Analysis of blood samples of myocardial infarction (MI) patients and controls showed elevated levels of H2S in MI patients (28 μM ± 1.111) vs. controls (23 μM ± 1.036) at p = 0.0015.