Abstract
Calcium transients drive cells to discharge prostaglandin E(2) (PGE(2)). We visualized PGE(2)-induced protein kinase A (PKA) activation and quantitated PGE(2) secreted from a single cell by combining fluorescence microscopy and a simulation model. For this purpose, we first prepared PGE(2)-producer cells that express either an optogenetic or a chemogenetic calcium channel stimulator: OptoSTIM1 or Gq-DREADD, respectively. Second, we prepared reporter cells expressing the Gs-coupled PGE(2) reporter EP2 and the PKA biosensor Booster-PKA, which is based on the principle of Förster resonance energy transfer (FRET). Upon the stimulation-induced triggering of calcium transients, a single producer cell discharges PGE(2) to stimulate PKA in the surrounding reporter cells. Due to the flow of the medium, the PKA-activated area exhibited a comet-like smear when HeLa cells were used. In contrast, radial PKA activation was observed when confluent MDCK cells were used, indicating that PGE(2) diffusion was restricted to the basolateral space. By fitting the radius of the PKA-activated area to a simulation model based on simple diffusion, we estimated that a single HeLa cell secretes 0.25 fmol PGE(2) upon a single calcium transient to activate PKA in more than 1000 neighboring cells. This model also predicts that the PGE(2) discharge rate is comparable to the diffusion rate. Thus, our method quantitatively envisions that a single calcium transient affects more than 1000 neighboring cells via PGE(2).Key words: prostaglandin E(2), imaging, intercellular communication, biosensor, quantification.