Multiple DNA damages induced by water radiolysis demonstrated using a dynamic Monte Carlo code

利用动态蒙特卡罗代码演示了水辐射分解引起的多种DNA损伤。

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Abstract

Multiple DNA damage resulting from different nearby ionizations of water molecules is an important process of the initial step of radiobiological effects. Several important characteristics of the damaged DNA site such as the critical size and types of chemical lesions are not well-known. We investigated this long-term issue by developing a dynamic Monte Carlo code for the chemical process. The reaction probabilities and the spatial distribution of lesions were theoretically solved as a function of the spur radius and distance between DNA and the initial ionisation position. From our previous reported results, we suggest that a hydroxyl radical and a hydrated electron from a single spur can concomitantly react within a 10 base pairs DNA to induce a multiple DNA damage site comprising a DNA single-strand break and reductive nucleobase damage; however, the reaction probability is 0.4% or less. Once this combination arises, it may result in a DNA double-strand break (DSB). DSBs are difficult to repair, which may lead to cell death or misrepair, and could lead to point mutations in the genome.

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