Spatio-temporal remodelling of the composition and architecture of the human ovarian cortical extracellular matrix during in vitro culture

体外培养过程中人卵巢皮质细胞外基质组成和结构的时空重塑

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作者:Johanne Grosbois, Emily C Bailie, Tom W Kelsey, Richard A Anderson, Evelyn E Telfer

Methods

Primordial follicle activation, stromal cell density, and ECM-related protein (collagen, elastin, fibronectin, laminin) positive area in the entire cortex were quantified at each time point using histological and immunohistological analysis. Collagen and elastin content, collagen fibre characteristics, and follicle distribution within the tissue were further quantified within each layer of the human ovarian cortex, namely the outer cortex, the mid-cortex, and the cortex-medulla junction regions. Main

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