Abstract
Precise design and control of molecular self-assembly as living creatures are exciting ideas in the field of nanotechnology. Characterized with predesigned geometries and accurate spatial addressability, programmable DNA origami nanostructures have been recognized as optimized tools for assembling multiple functional components. A variety of biomolecules can be attached to the nanoscale drawing boards in a site-specific fashion, thus facilitating the precise construction of DNA origami-based materials for studies on biological interface. In this minireview, we highlight the recent advances in the precise construction of DNA origami-based materials with artificial bio-structures and/or biomimicking functions. The regulation of biological functions by these DNA origami-engineered assemblies at the bio-interface has been summarized and discussed.