Abstract
Five years after a German study on insect biomass described a multi-decade decline in nature protected habitats, the DINA (Diversity of Insects in Nature protected Areas) project has investigated the status of insects in 21 selected nature reserves across Germany in the years 2020 and 2021. We used the same methods and protocols for trapping and measuring the biomass of flying insects as in the earlier study. Across two vegetation periods, we accumulated a comprehensive data set of 1621 data points of two-week emptying intervals to evaluate the insect biomass along gradients from arable land into nature reserves through transects of Malaise traps. On average, we observed an increase in maximum insect biomass per day along the transect from the edge to the centre of the nature reserve. Overall, the measured insect biomass remained at low levels, consistent with previous findings from the years 2007-2016. There were no significant regional differences. The results show that protected habitats have higher insect biomass compared to farmland and are therefore essential for insects but are unlikely to be sufficient to sustain insect biodiversity. Further measures need to be taken for better protection and sustainment of insects, which fulfil key functions in all terrestrial ecosystems.