Abstract
The family Diatrypaceae is a less well-known group within the order Xylariales (Ascomycota). Initially, the focus on its metabolites was related to the pathogenicity of one of its members, Eutypa lata. To date, a total of 254 natural products have been identified from Diatrypaceae strains. These compounds include terpenoids, sterols, polyketones, phenols, and acetylene aromatic compounds, which have shown anticancer, cytotoxic, anti-inflammatory, antimicrobial, and antiviral activities. The complex and diverse structural types, along with the diverse bioactivities, highlight the potential of Diatrypaceae as a valuable source of bioactive natural products. In this review, a deep analysis of the biosynthesis of pimarane diterpenes and scoparasin-type cytochalasins is provided, coupled with a compilation of the biosynthetic pathways of aromatic acetylene compounds in filamentous fungi. This comprehensive review not only enhances our understanding of the natural product chemistry, biological activities, and biosynthesis of secondary metabolites from the Diatrypaceae family but also promotes the exploitation and development of important bioactive compounds and potential strains.