Pneumocystis carinii pneumonia in thoroughbred foals: identification of a genetically distinct organism by DNA amplification

纯种马驹卡氏肺囊虫肺炎:通过DNA扩增鉴定出一种基因不同的病原体

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Abstract

Genetically distinct forms of Pneumocystis carinii infect several mammalian hosts. We report the amplification of P. carinii DNA from samples of two infected thoroughbred foal lungs by using primers designed from the sequence of a P. carinii mitochondrial rRNA gene; these primers also prime the amplification of P. carinii DNA from other hosts. The nucleotide sequence of part of the mitochondrial rRNA gene amplified from P. carinii infecting one of the foals was determined and found to be distinct from that of published rat-, rabbit-, ferret-, and human-derived P. carinii sequences.

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