Conclusion
This research enabled purification of human heat shock protein by a laboratory prepared column chromatography.
Methods
Initially the human Hsp90β gene was cloned into the heat inducible expression vector pGP1-2 and then the recombinant protein was isolated by ion exchange chromatography. After intradermal injection of confirmed purified band of protein to rabbits and isolation of the serum IgG antibody, for its affinity purification, the rabbit's purified Hsp90 specific IgG was coupled to the cyanogen bromide-activated Sepharose 4B.
Results
The recovery of the purified protein of interest by affinity chromatography was 50%.