Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

Hin重组酶与DNA hix位点结合的结构元件预测

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Abstract

Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

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