Abstract
BACKGROUND: Phytogenic additives would be helpful to alleviate the adverse effect of Campylobacter jejuni on the performance and physiological responses of broiler chickens. OBJECTIVE: This experiment was carried out to investigate the effects of Echinacea purpurea (EP) and Thymbra spicata (TS) on the performance, nutrient digestibility, serum biochemistry, intestinal morphology, intestinal microbiota and immune responses of broilers challenged with C. jejuni from 0 to 42 days of age. METHODS: A total of 240 male broiler chickens were divided into 6 groups and fed various diets: a control diet; the control diet supplemented with EP at 0.25% (EP25) or 0.50% (EP50); the control diet supplemented with TS at 0.25% (TS25) and 0.50% (TS50); or the control diet containing erythromycin at 55 ppm. Chicks were orally challenged with C. jejuni on Days 21 and 23 of age. RESULTS: EP and TS25 diets enhanced European production efficiency factor, feed conversion ratio and digestibility of dry matter and organic matter. TS25 increased duodenal villous height (VH) and surface area on Day 42 of age. EP25 diet increased ileal VH compared to control and erythromycin diets. Diets containing certain EP25 and TS increased the Bifidobacterium population and decreased C. jejuni population on Day 39 of age. EP50 and TS50 diets increased antibody titration against Newcastle disease virus. CONCLUSIONS: In conclusion, EP and TS dietary supplementation improved performance, microflora, intestinal morphology and immune responses in C. jejuni-challenged broilers.