Chromatin very small angle neutron scattering: further evidence for a 30 nm diameter super coil in dilute solutions

染色质超小角中子散射:稀溶液中直径为 30 nm 的超螺旋结构的进一步证据

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Abstract

Intact chromatin, chromatin minus histone H1, and nuclease digestion fragments have been studied by very small angle neutron scattering. The results are not consistent with a straight chain of nucleosomes and require the presence of a higher order coiling in monovalent salt solutions. The data are interpretable by a structure having a cross section radius of gryration of 8.5 +/- 1 nm, which suggests an outer diameter for a coil of nucleosomes of 27 +/- 3 nm.

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