Abstract
Deep learning algorithms are powerful tools to analyse, restore and transform bioimaging data, increasingly used in life sciences research. These approaches now outperform most other algorithms for a broad range of image analysis tasks. In particular, one of the promises of deep learning is the possibility to provide parameter-free, one-click data analysis achieving expert-level performances in a fraction of the time previously required. However, as with most new and upcoming technologies, the potential for inappropriate use is raising concerns among the biomedical research community. This perspective aims to provide a short overview of key concepts that we believe are important for researchers to consider when using deep learning for their microscopy studies. These comments are based on our own experience gained while optimising various deep learning tools for bioimage analysis and discussions with colleagues from both the developer and user community. In particular, we focus on describing how results obtained using deep learning can be validated and discuss what should, in our views, be considered when choosing a suitable tool. We also suggest what aspects of a deep learning analysis would need to be reported in publications to describe the use of such tools to guarantee that the work can be reproduced. We hope this perspective will foster further discussion between developers, image analysis specialists, users and journal editors to define adequate guidelines and ensure that this transformative technology is used appropriately.