Separation of protein oligomers by blue native gel electrophoresis

利用蓝色天然凝胶电泳分离蛋白质寡聚体

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Abstract

Native gel electrophoresis is used as a tool to assess structural differences in proteins. This article presents an application to separate oligomeric forms of proteins such as human immunodeficiency virus type 1 (HIV-1) reverse transcriptase monomers and homodimers. Technical difficulties encountered with various native gel techniques and ways to circumvent them are described.

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