Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis

利用聚丙烯酰胺凝胶电泳分离十个呼肠孤病毒基因组片段

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Abstract

Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 x 10(6) daltons, respectively, or a total of 15 x 10(6) daltons.

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