A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels

一种制备低pH十二烷基硫酸钠/聚丙烯酰胺梯度凝胶的方法

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Abstract

1. A low-pH lithium dodecyl sulphate/polyacrylamide-gradient slab-gel system, suitable for electrophoresis, is described, and the migration properties of standard proteins are compared on this and conventional high-pH gels. 2. Cytochrome oxidase may be partially resolved into its component polypeptides. The order of migration of these is, however, dependent on the pH of the gel system.

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