Identification of some bacteriophage T4 prereplicative proteins on two-dimensional gel proteins

在二维凝胶上鉴定一些T4噬菌体复制前蛋白

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Abstract

Profiles of bacteriophage T4 early proteins resolved by a two-dimensional nonequilibrium pH gradient electrophoresis system (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell, Cell 12:1133--1142, 1977) are presented. Over 65 phage-induced proteins were resolved. Amber or deletion mutants were used to identify 17 proteins in the gel patterns as the products of specific genes.

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