Nucleotide sequencing of DNA: preliminary characterization of the products of specific cleavages at guanine, cytosine, or adenine residues (bacteriophage M13-ribosubstitution-DNA polymerase I-electrophoresis-two-dimensional fingerprinting)

DNA核苷酸测序:鸟嘌呤、胞嘧啶或腺嘌呤残基特异性切割产物的初步表征(噬菌体M13-核糖体置换-DNA聚合酶I-电泳-二维指纹图谱)

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Abstract

DNA synthesized in vitro from a phage M13 template has been cleaved at either guanine, adenine, or cytosine residues by ribosubstitution techniques. Fingerprints of the fragments obtained suggest that DNA sequencing will be possible with this technique.

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