Abstract
Phycobilisomes were isolated from a Nostoc sp. strain Mac in phosphate buffer (pH 7.0) by treatment with 1% Brij 56 and centrifugation on discontinuous sucrose gradients (2.0, 1.0, 0.5, and 0.25 M in the proportions 6:4:4:10 ml, respectively). Absorption spectra of isolated phycobilisomes showed the presence of phycoerythrin, phycocyanin, and allophycocyanin. The phycobilisome pigments were partially resolved by electrophoresis on acrylamide gels. Stained gels demonstrated that each main protein band corresponded to a pigmented region. The phycobilisomes appeared compact with a rounded surface and flattened base (about 40-nm diameter) at the attachment site to the photosynthetic lamellae. Fixation in glutaraldehyde caused a significant reduction in total pigment absorption, as well as shifts in the absorption maxima, particularly that of phycoerythrin.