Aim
In this paper, the authors report on the development of a biosensor based on a miniature, integrated optical Mach-Zehnder interferometer. Method: Functionalization of the measuring arm of the sensor by antibodies, made the rapid and specific label-free detection of pathogens feasible.
Conclusions
This makes the newly developed biosensor a promising device for a wide range of applications, not only in medical microbiology, but microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies as well.
Results
Using the combination of the interferometer with a microfluidic system, the device was able to detect Escherichia coli bacteria at concentrations as low as 10(6) colony forming unit/ml within minutes. Conclusions: This makes the newly developed biosensor a promising device for a wide range of applications, not only in medical microbiology, but microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies as well.