Different mutation profiles between epithelium and stroma in endometriosis and normal endometrium

子宫内膜异位症和正常子宫内膜的上皮和基质之间的不同突变谱

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作者:Kazuaki Suda, Hirofumi Nakaoka, Kosuke Yoshihara, Tatsuya Ishiguro, Sosuke Adachi, Hiroaki Kase, Teiichi Motoyama, Ituro Inoue, Takayuki Enomoto

Methods

The laser microdissection method was used to obtain stromal cells in ovarian endometriotic and normal endometrial tissues from patients with ovarian endometriosis and/or other non-invasive gynecological diseases. Target gene sequencing was performed to assess and compare the mutation profiles of stromal cells with those of epithelial cells obtained in our previous study. For target gene sequencing, 76 genes were selected based on previous genomic analyses for ovarian endometriosis, normal endometrium, endometriosis-related ovarian cancer and endometrial cancer. Main

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