Prevalence, Genetic Structure, and Antifungal Susceptibility of the Cryptococcus neoformans/C. gattii Species Complex Strains Collected from the Arboreal Niche in Poland

从波兰树栖生态位收集的新型隐球菌/格特隐球菌复合菌株的流行率、遗传结构和抗真菌敏感性

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作者:Magdalena Florek, Agnieszka Korzeniowska-Kowal, Anna Wzorek, Katarzyna Włodarczyk, Maja Marynowska, Aleksandra Pogorzelska, Maria Brodala, Sebastian Ploch, Daniel Buczek, Katarzyna Balon, Urszula Nawrot

Abstract

Fungi belonging to the Cryptococcus neoformans/C. gattii species complex (CNGSC) are etiological agents of serious and not infrequently fatal infections in both humans and animals. Trees are the main ecological niche and source of potential exposition concerning these pathogens. With regard to epidemiology of cryptococcosis, various surveys were performed worldwide, enabling the establishment of a map of distribution and genetic structure of the arboreal population of the CNGSC. However, there are regions, among them Central and Eastern Europe, in which the data are lacking. The present study shows the results of such an environmental study performed in Wrocław, Poland. The CNGSC strains were detected in 2.2% of the tested trees belonging to four genera. The obtained pathogen population consisted exclusively of C. neoformans, represented by both the major molecular type VNI and VNIV. Within the tested group of isolates, resistance to commonly used antimycotics was not found, except for 5-fluorocytosine, in which about 5% of the strains were classified as a non-wild type.

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