Abstract
The mitogenome of Pseudolimnophila (Pseudolimnophila) brunneinota was sequenced, the new representative of the subfamily Limnophilinae in mitogenome. The completed mitogenome is 15,985 bp totally, comprising of 13 protein-coding genes, 2 rRNAs, 22 transfer RNAs and control region. All genes have the similar locations and strands with other published species of Tipuloidea. The nucleotide composition biases toward A and T, which together made up 78.00％of the entirety. Bayesian inference analysis strongly supported the monophyly of Chioneinae + Limnophilinae. It suggested that the phylogenetic relationship within Tipuloidea is Pediciidae + (Limoniidae ((Chioneinae + Limnophilinae)+ Limoniinae) + (Tipulidae + Cylindrotomidae)).