Conclusions
Hsa_circ_0001017 suppressed the growth and metastasis and increased cell apoptosis of glioma, and this effect was associated with hsa-let-7g-3p/NDST3 axis.
Material and methods
Hsa_circ_0001017 expression was analysed in glioma tissue and cells using qRT-PCR assay. Flow cytometer, Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell were used to analyse glioma cell apoptosis, viability, colony-forming ability, migration, and invasion. The expression of N-deacetylase and N-sulfotransferase 3 (NDST3) and epithelial-mesenchymal transition (EMT)-related proteins was measured using western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assay were performed to determine the target relationship.
Methods
Hsa_circ_0001017 expression was analysed in glioma tissue and cells using qRT-PCR assay. Flow cytometer, Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell were used to analyse glioma cell apoptosis, viability, colony-forming ability, migration, and invasion. The expression of N-deacetylase and N-sulfotransferase 3 (NDST3) and epithelial-mesenchymal transition (EMT)-related proteins was measured using western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assay were performed to determine the target relationship.
Results
In glioma tissues and cells, hsa_circ_0001017 expression was decreased. The overexpression of hsa_circ_0001017 inhibited glioma cell proliferation, EMT, migration, and invasion and promoted glioma cell apoptosis, while the knockdown of hsa_circ_0001017 caused the opposite results. Mechanistically, hsa_circ_0001017 sponged hsa-let-7g-3p and NDST3 was the target gene of hsa-let-7g-3p. Moreover, the tumour-suppressive role of circ_0001017 was associated with hsa-let-7g-3p and NDST3. Conclusions: Hsa_circ_0001017 suppressed the growth and metastasis and increased cell apoptosis of glioma, and this effect was associated with hsa-let-7g-3p/NDST3 axis.