Abstract
A Gram-stain-positive, aerobic, white-coloured, rod-shaped bacteria, designated as a strain dW9(T), was isolated from soil. Strain dW9(T) was catalase-positive and oxidase-negative. Strain dW9(T) grew at temperature of 20-37°C and at pH of 5.0-7.0. Phylogenetic and 16S rRNA gene analysis indicated that strain dW9(T) belonged to the genus Paenibacillus with its closest relative being Paenibacillus filicis S4(T) (97.4% sequence similarity). The genome size of dW9(T) was 7,787,916 bp with DNA G+C content of 51.3%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of dW9(T) with its closest relatives were found to be <22.0% and <74.0%, respectively. The only respiratory quinone was MK-7, and the major fatty acids were antiso-C(15:0) and iso-C(16:0). Overall, the comprehensive taxonomic analysis revealed that strain dW9(T) met all the fundamental criteria to be classified as a novel species within the genus Paenibacillus. Accordingly, we propose the name Paenibacillus gyeongsangnamensis sp. nov., with the type strain dW9(T) (=KCTC 43431(T) =NBRC 116022(T)).