Analysis of tRNA(Cys) processing in the absence of CCAase in Bacillus subtilis

In Bacillus subtilis, the tRNA(Cys) lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNA(Cys) processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNA(Cys).