Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

器官映射抗体组:标准化多重组织成像的社区资源

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作者:Ellen M Quardokus #, Diane C Saunders #, Elizabeth McDonough, John W Hickey, Christopher Werlein, Christine Surrette, Presha Rajbhandari, Anna Martinez Casals, Hua Tian, Lisa Lowery, Elizabeth K Neumann, Frida Björklund, Taruna V Neelakantan, Josh Croteau, Anne E Wiblin, Jeremy Fisher, April J Liven

Abstract

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

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