Background
The enteric nervous system controls gastrointestinal functions such as secretion and smooth muscle contraction/relaxation. Neuronal enteric dysfunction is a feature of many direct gastrointestinal disorders and can be secondary to central nervous system disorders. Research in this field has been limited and there are few published
Conclusion
We provide a novel method for researchers to dissociate and grow high quality enteric neuronal cultures. Our method can be used for studies on gastrointestinal diseases caused by enteric neuronal dysfunction and to explore possible pharmacological interventions in vitro.
Methods
Unlike other protocols our method does not require a time consuming challenging dissection, long enzymatic treatment times or the use of specialized equipment. Resulting cultures are of higher quality and can be sustained longer permitting proper neuronal recovery. In addition cell attachment to culture substrates have been optimized.
Results
This protocol produces a high yield of neuronal cells. Multiple neuronal subtypes reflecting the in vivo population are observed. Cultures are optimal at 3 weeks in vitro but can be sustained for at least 5 weeks. Comparison with existing methods: Unlike other protocols our method does not require a time consuming challenging dissection, long enzymatic treatment times or the use of specialized equipment. Resulting cultures are of higher quality and can be sustained longer permitting proper neuronal recovery. In addition cell attachment to culture substrates have been optimized.