Abstract
Colorectal cancer (CRC) is one of the leading causes of cancer deaths worldwide, CRC was estimated to be the third most commonly diagnosed cancer and a leading cause of cancer deaths in developed countries due to therapy resistance and metastasis. Cancer stem cells (CSCs) were found in a variety of malignant tumors, including colorectal cancer. miR-27b play pivotal roles in the acquisition of CSC properties such as tumor initiation, drug resistance and asymmetric cell division. The aim of the present study was to investigate the underlying mechanisms that miR-27b inhibits proliferation, invasion and migration of CSCs. In present study, miR-27b were found to be significantly upregulated in CSCs. Overexpression of miR-27b inhibit CSCs proliferation and migration. In addition, overexpression of miR-27b suppress the character expression of CSCs, including of CD44, CD133, Sox2 and Oct4. Furthermore, it has been demonstrated that miR-27b is directly targeted by PIK3CA and miR-27b overexpression can effectively attenuate the expression of Phosphor-PI3K p110α and phosphor-Akt. In conclusion, these results reveal that PIK3CA is significantly downregulated by miR-27b expression in CSCs. Thus, we presume that miR-27b may be a therapeutic anti-tumor agent for CRC via targeting PI3K p110α.