Abstract
Porcine circovirus 2 (PCV2) has been proved to increase the risk of other pathogens infection through antagonizing the host type I interferon (IFN) response. Previously, we have reported that PCV2 infection efficiently inhibits type I interferon production induced by other DNA viruses. However, whether PCV2 can inhibit type I interferon signaling is less reported. Herein, we found that PCV2 interfered with the activation of IFN signaling pathway, which led to a significantly reduced IFN-stimulated genes (ISGs) transcription after IFN-α stimulation both in vivo and in vitro. In PCV2-infected cells, IFN-induced tyrosine phosphorylation of STAT1 and STAT2 and their heterodimerization were decreased. Meanwhile, the nuclear translocation of phosphorylated STAT1/STAT2 was also decreased. Based on these findings, we further determined that roles of PCV2 Cap and Rep in the suppression of IFN-I signaling, and found that Cap acted as a predominant regulator in the early phase infection. PCV2 Cap could significantly reduce the phosphorylation of STAT1 and STAT2, the nuclear translocation of phosphorylated STAT1/STAT2, and IFN-stimulated response element (ISRE) promoter activity, results in a decreased ISGs transcription. As the binding protein of PCV2 Cap, gC1qR protein was also involved in this inhibition process. Knockdown of gC1qR could alleviate the inhibitory effects of either PCV2 infection or Cap on the activation of IFN signaling. These findings demonstrated that PCV2 infection interferes with the activation of type I IFNs signaling pathway depending on its Cap and host gC1qR protein.