Abstract
Hydroxyl radicals (·OH) offer exceptional potential for cancer treatment through reactive oxygen species (ROS) amplification and apoptotic induction. However, conventional Fe-based metal-organic framework (Fe-MOF) nanomaterials are limited by inadequate Fe(2+) concentrations, resulting in suboptimal Fenton catalytic performance. This study presents concave octahedral Fe-MOF nanomaterials with integrated bimetallic Fe/Zn centers through controlled solvothermal synthesis. The nanoplatform exhibits high specific surface area (559 m(2)/g) and 5-fluorouracil (5-FU) loading efficiency (58.7%). These structural properties establish it as a potential nanobuilding block for constructing stimuli-responsive gels. With optimized Fe(2+) content (57.3%), the Fe-MOF material shows enhanced nanozyme-like activity (V(max) = 4.58 × 10(-7) M/s, K(cat) = 1.83 × 10(-3) s(-1)) for H(2)O(2)-mediated ·OH generation. The Fe-MOF@FU demonstrates pH-responsive drug release (76.5% at pH 5.0) and glutathione (GSH) depletion, synergistically enhancing oxidative stress. Biocompatibility studies confirm safety, while in vitro investigations show remarkable anticancer activity against 4T1 cells with 17.8% viability, supporting its dual role as an independent therapeutic agent and a functional component for future gel-based delivery systems.