Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining

使用液基细胞学和 WT1 免疫酶染色对人尿中的足细胞和壁层上皮细胞进行量化

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作者:Hiroyuki Ohsaki, Toru Matsunaga, Taishi Fujita, Yasunori Tokuhara, Shingo Kamoshida, Tadashi Sofue

Abstract

In glomerular disease, podocytes and parietal epithelial cells (PECs) are shed in the urine. Therefore, urinary podocytes and PECs are noninvasive biomarkers of glomerular disease. The purpose of this protocol is to employ immunocytochemistry to detect podocytes and PECs, using the WT1 antibody on liquid-based cytology slides.

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