Abstract
Acellular dermal matrices (ADMs) are increasingly used for the restoration of soft-tissue defects of the oral cavity due to their ability to facilitate faster healing and reduce scar formation without rejection. However, few studies have focused on the histopathology and biological mechanisms involved in their use. The aim of the present study was to observe tissue growth, histopathologic changes and altered biomolecular signatures that occur during the repair of oral defects in rabbit models over time, either with or without the employment of ADM. Animals were sacrificed 1, 2 and 4 weeks following surgery and histological changes were evaluated using hematoxylin and eosin staining. Reverse transcription-polymerase chain reaction and western blot analysis were used to determine changes in the expression of vascular endothelial growth factor (VEGF) and glucose transporter 1 (GLUT1). It was demonstrated that wounds treated with ADM exhibited a weak inflammatory reaction and faster epithelialization and revascularization compared with untreated wounds. This may have been caused by the elevated levels of VEGF and GLUT1 protein detected in the ADM-treated defects. Thus, treating wounds of the oral mucosa with an ADM improves pathological responses compared with those with an untreated wound. The current study demonstrates the underlying mechanisms by which ADM promotes wound healing in defects of the oral mucosa and the results provide further evidence for the use of ADM in clinical settings for the repair of mucosal defects.