Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

拟南芥中水杨酸 (SA) 和 SA-葡萄糖苷的定量分析

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作者:Valérie Allasia, Benoit Industri, Michel Ponchet, Michaël Quentin, Bruno Favery, Harald Keller

Abstract

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.

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