Abstract
The transmembrane alkane monooxygenase AlkB and rubredoxin AlkG form an electron transfer complex that hydroxylates terminal alkanes to produce alcohols. The recent cryoEM study of Fontimonas thermophila AlkB-AlkG complex (FtAlkBG) revealed its architecture, including a dodecane (D12) substrate in the diiron active site. However, the molecular mechanism of action of FtAlkBG remains unknown. Here, we examined the FtAlkBG dynamics and interactions by multiscale computations, including molecular dynamics (MD) simulations, elastic network model (ENM), and QM/MM study of the oxidative mechanism at the catalytic site of AlkB. D12 remained stably bound to the catalytic site during MD runs, coordinated by hydrophobic residues I267, L263, L264, and I133, critical to substrate stabilization. MD simulations further showed that D12 could exit the catalytic site via a well-defined translocation pathway gated by I54, S49, and F46, through two intermediate states before nearly leaving the protein, and diffuse back to the active site. Several salt bridges and conserved AlkB-AlkG contacts were enhanced upon substrate binding. Substrate binding also mediated the association/dissociation events required for efficient electron transfer. It promoted tighter coupling between the diiron center in AlkB and the iron in AlkG. The allosteric effects relevant to enzymatic activity regulated by the substrate binding and channeling were further delineated by ENM analysis which confirmed a strong coupling spanning the site of entry from the membrane, the catalytic site and the AlkB-AlkG interface. Our study provides new insights into key sites that could be targeted for developing AlkB-variants with desirable alkane conversion functions.