Galangin exosomes induce cell apoptosis through miR-10b/P53 axis in gastric cancer

高良姜素外泌体通过 miR-10b/P53 轴诱导胃癌细胞凋亡

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Abstract

Galangin is an extract of traditional Chinese medicine with anti-cancer effect. Exosomes (Exo) contain RNA, peptides, or proteins and communicate between cells through a bio-genesis pathway. In the present study, Gastric cancer (GC) cell exosomes containing galangin were obtained. MicroRNAs (miRNAs) in exosomes containing galangin (galangin-Exo) were screened by RNA-seq. GC cells were treated with galangin-Exo and the changes of cell proliferation were analyzed. Besides, the mouse model of GC was established to verify the effect of galangin-Exo in vivo. The results showed that galangin-Exo inhibited the growth of AGS and BGC823 cells. Additionally, RNA-seq data showed that miR-10b-5p was differentially expressed in exosomes. The reduced expression of miR-10b-5p which increased the expression of P53 inhibited the growth and induced apoptosis of GC cells. The in vivo results confirmed that exosomes can inhibit tumor growth by delivering galangin. In conclusion, our results suggest that galangin-Exo have anti-cancer effect on GC and can be used as a potential therapeutic strategy.

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